Manure application can spread antimicrobial resistance (AMR) from manure to soil and surface water. This study evaluated the role of the soil texture on the dynamics of antimicrobial resistance genes (ARGs) in soils and surrounding surface waters. Six dairy farms with distinct soil textures (clay, sand, and peat) were sampled at different time points after the application of manure, and three representative ARGs sul1, erm(B), and tet(W) were quantified with qPCR. Manuring initially increased levels of erm(B) by 1.5 ± 0.5 log copies/kg of soil and tet(W) by 0.8 ± 0.4 log copies/kg across soil textures, after which levels gradually declined. In surface waters from clay environments, regardless of the ARG, the gene levels initially increased by 2.6 ± 1.6 log copies/L, after which levels gradually declined. The gene decay in soils was strongly dependent on the type of ARG (erm(B) < tet(W) < sul1; half-lives of 7, 11, and 75 days, respectively), while in water, the decay was primarily dependent on the soil texture adjacent to the sampled surface water (clay < peat < sand; half-lives of 2, 6, and 10 days, respectively). Finally, recovery of ARG levels was predicted after 29–42 days. The results thus showed that there was not a complete restoration of ARGs in soils between rounds of manure application. In conclusion, this study demonstrates that rather than showing similar dynamics of decay, factors such as the type of ARG and soil texture drive the ARG persistence in the environment.
MULTIFILE
Application of animal manure to soils results in the introduction of manure-derived bacteria and their antimicrobial resistance genes (ARGs) into soils. ResCap is a novel targeted-metagenomic approach that allows the detection of minority components of the resistome gene pool without the cost-prohibitive coverage depths and can provide a valuable tool to study the spread of antimicrobial resistance (AMR) in the environment. We used high-throughput sequencing and qPCR for 16S rRNA gene fragments as well as ResCap to explore the dynamics of bacteria, and ARGs introduced to soils and adjacent water ditches, both at community and individual scale, over a period of three weeks. The soil bacteriome and resistome showed strong resilience to the input of manure, as manuring did not impact the overall structure of the bacteriome, and its effects on the resistome were transient. Initially, manure application resulted in a substantial increase of ARGs in soils and adjacent waters, while not affecting the overall bacterial community composition. Still, specific families increased after manure application, either through the input of manure (e.g., Dysgonomonadaceae) or through enrichment after manuring (e.g., Pseudomonadaceae). Depending on the type of ARG, manure application resulted mostly in an increase (e.g., aph(6)-Id), but occasionally also in a decrease (e.g., dfrB3) of the absolute abundance of ARG clusters (FPKM/kg or L). This study shows that the structures of the bacteriome and resistome are shaped by different factors, where the bacterial community composition could not explain the changes in ARG diversity or abundances. Also, it highlights the potential of applying targeted metagenomic techniques, such as ResCap, to study the fate of AMR in the environment.
MULTIFILE
The impact of the construction industry on the natural environment is severe, natural areas are changedinto predominantly hard solid surfaces, the energy use in the built environment is high and the industryputs huge claims on materials.
MULTIFILE
Plastic products are currently been critically reviewed due to the growing awareness on the related problems, such as the “plastic soup”. EU has introduced a ban for a number of single-use consumer products and fossil-based polymers coming in force in 2021. The list of banned products are expected to be extended, for example for single-use, non-compostable plastics in horticulture and agriculture. Therefore, it is crucial to develop sustainable, biodegradable alternatives. A significant amount of research has been performed on biobased polymers. However, plastics are made from a polymer mixed with other materials, additives, which are essential for the plastics production and performance. Development of biodegradable solutions for these additives is lacking, but is urgently needed. Biocarbon (Biochar), is a high-carbon, fine-grained residue that is produced through pyrolysis processes. This natural product is currently used to produce energy, but the recent research indicate that it has a great potential in enhancing biopolymer properties. The biocarbon-biopolymer composite could provide a much needed fully biodegradable solution. This would be especially interesting in agricultural and horticultural applications, since biocarbon has been found to be effective at retaining water and water-soluble nutrients and to increase micro-organism activity in soil. Biocarbon-biocomposite may also be used for other markets, where biodegradability is essential, including packaging and disposable consumer articles. The BioADD consortium consists of 9 industrial partners, a branch organization and 3 research partners. The partner companies form a complementary team, including biomass providers, pyrolysis technology manufacturers and companies producing products to the relevant markets of horticulture, agriculture and packaging. For each of the companies the successful result from the project will lead to concrete business opportunities. The support of Avans, University of Groningen and Eindhoven University of Technology is essential in developing the know-how and the first product development making the innovation possible.
The pipelines are buried structures. They move together with the soil during a seismic event. They are affected from ground motions. The project aims to find out the possible effects of Groningen earthquakes on pipelines of Loppersum and Slochteren.This project is devised for conducting an initial probe on the available data to see the possible actions that can be taken, initially on these two pilot villages, Loppersum and Slochteren, for detecting the potential relationship between the past damages and the seismic activity.Lifeline infrastructure, such as water mains and sewerage systems, covering our urbanised areas like a network, are most of the times, sensitive to seismic actions. This sensitivity can be in the form of extended damage during seismic events, or other collateral damages, such as what happened in Christchurch Earthquakes in 2011 in New Zealand when the sewerage system of the city was filled in with tonnes of sand due to liquefaction.Regular damage detection is one of key solutions for operational purposes. The earthquake mitigation, however, needs large scale risk studies with expected spatial distribution of damages for varying seismic hazard levels.
In the Netherlands, the Agri-Food and Water Top Sectors aim at climate neutral food systems that close loops in the food value chains from farm to fork, based on efficiency of natural resource management, optimum use of food, a reduced use of natural resources and less environmental pressure, and optimum use of residue streams. It is also in their ambitions to promote and market Dutch circular solutions in foreign countries, such as emergent economies. The transition to a circular economy in the food chain in emergent economies requires a radical transformation, in which an integrated approach is required. In this regard, Indonesia strives for green development representing an advantageous market opportunity for Dutch SME’s offering circular innovations on the food value chain. The consortium in this project would like to explore the opportunities for applying integrated approaches contributing to the transition to a circular economy in the food chain of emerging countries, in this case Indonesia, that could open market opportunities in the agri-food sector. The integrated approach includes innovations on effective use of natural resources (e.g., soil and water), innovations on ‘reshaping’ local organization and governance, and innovations on food/streams value chains.
