Search results

Control of anthracnose (Colletotrichum gloeosporioides) growth in “Hass” avocado fruit using sachets filled with oregano oil-starch-capsules.

Active antifungal packaging is a technological solution for reducing the postharvest losses of fruits and vegetables associated with phytopathogens. Anthracnose (Colletotrichum gloeosporioides) is the principal fungus that causes post-harvest avocado fruit decay. In this study, antifungal sachets filled with oregano oil-starch capsules were prepared, and their active effects were demonstrated on Hass avocado fruits. Oregano oil (31 % of carvacrol) was encapsulated with corn starch by spray drying. Tyvek sachets (4 × 4 cm) filled with 80 (T1) and 160 mg (T2) of oregano oil-starch capsules (99.35 ± 1.86 mg g − 1) were fabricated. The antifungal effects of the sachets were tested in vitro and in vivo using a humidity chamber (90–95 % relative humidity (RH)) on fruits inoculated with anthracnose. The results showed that T1 and T2 inhibited 75.21 ± 2.81 and 100 % in vitro growth of anthracnose at 25 °C for 12 days. Furthermore, Hass avocado fruits stored in a humidity chamber at 25 °C for 6 days showed that only T2 significantly (p < 0.05) reduced the area of lesion produced by artificial inoculation of Hass avocado fruits with anthracnose. On average, the lesion area in the Hass avocado fruits treated with T2 was 13.94 % smaller than that in the control fruit.

An ecosystem services analysis of oil palm and alternative land use systems on peat in Malaysia

In this article, we assess the potential of alternative land use systems using non-drainage peatland species which could eventually phase out or partly replace oil palm plantations on undrainable peatlands. We have used the ecosystem services approach to analyse what scenarios using drainage-free peatland species could be suitable alternatives for oil palm cultivation on peat and how these scenarios compare to oil palm plantations in terms of selected ecosystem services. Our results indicate that alternative paludiculture systems will provide more direct and indirect ecosystem services than oil palm plantations on peat. We also found that stakeholders were aware of issues with growing oil palm on peat, and that there was a general intention for sustainable use of peatlands amongst several groups of stakeholders. Replacing oil palm with alternative systems such as paludiculture in Malaysia is not yet realistic. The most important impediments are a lack of knowledge on potential of non-drainage peatland species and its associated value chains, as well as the technical difficulty for smallholders to implement such a system. We recommend starting experimental plantings with paludiculture systems to further test species performance, life cycle analysis, growth, intercropping limitations and possibilities, yields and improvements in the value chain.

Control of mango decay using antifungal sachets containing of thyme oil/modified starch/agave fructans microcapsules

The aim of the present work was to control phytopathogens associated to mango decay by exposure to antifungal thyme oil-starch/agave fructans microcapsules in Nylon sachets. Fusarium pseudocircinatum, Alternaria alternata, Neofusicocum kwambonambiense, Cladosporium pseudocladosporioides, and Colletotrichum gloeosporioides were isolated from mango fruits and its mycelial growth was 100% inhibited with 5 μL of thyme oil. Thyme oil was microencapsulated with modified starch/agave fructans by spray drying. Thymol content, size, and shape of microcapsules were evaluated. Antifungal sachets were prepared by filling Nylon bags (4 × 4 cm) with different doses of thyme oil microcapsules: 0.10, 0.15 and 0.20 g. The mycelial growth of all microorganisms was controlled with 0.10, 0.15 and 0.20 g of antifungal sachets. The incidence (75 and 65%) and severity (77 and 41%) of C. gloeosporioides were effectively reduced in mango, respectively, using 0.10 and 0.20 g of antifungal sachets.

Supercritical fluid extraction of oil from Andean Lupin seeds

Pre-certification conditions of independent oil palm smallholders in Indonesia

Smallholders, who cultivate ±30% of the global palm oil land, are critical to the realization of a sustainable palm oil sector. However, particularly independent smallholders, untied to mills, lag behind in yields and experience challenges to market their produce. Sustainability certification, such as by the Roundtable on Sustainable Palm Oil (RSPO), is proposed as a way to improve smallholder livelihoods, while protecting the environment. However, independent smallholders experience barriers to obtain certification. Through interviews with 18 RSPOcertified independent smallholder groups in Indonesia and 9 certification facilitators, this study examines how pre-certification conditions regarding smallholders’ socio-economic backgrounds, legality, group organization, planation management practices, and local supply chain conditions impact prospects for RSPO certification, and how groups who successfully achieved certification have dealt with challenges during the certification process. We found that the majority (77%) of RSPO certified independent smallholders in Indonesia consists of ‘former scheme’ smallholders. These smallholders often have clear land legality and are organized in groups prior to certification, which increases their eligibility for RSPO certification. However, due to upfront and recurrent costs for certification, as well as complexities in meeting RSPO standards, access to certification is strongly dependent on external facilitators. To up-scale certification for independent oil palm smallholders, and include more nonscheme smallholders, certification projects should involve more local actors including local governments and certified smallholder groups. In addition, certification should focus on core social and environmental concerns for smallholders, while being flexible with regards to the forms of proof needed to fulfil legality requirements.

Vegetable oils as carbon and energy source for Aureobasidium melanogenum in batch cultivation

Dark homogenous fungal-based layers called biofinishes and vegetable oils are keyingredients of an innovative wood protecting system. The aim of this study was todetermine which of the vegetable oils that have been used to generate biofinishes onwood will provide carbon and energy for the biofinish-inhabiting fungus Aureobasidiummelanogenum, and to determine the effect of the oil type and the amount of oil on thecell yield. Aureobasidium melanogenum was cultivated in shake flasks with differenttypes and amounts of carbon-based nutrients. Oil-related total cell and colony-formingunit growth were demonstrated in suspensions with initially 1% raw linseed,stand linseed, and olive oil. Oil-related cell growth was also demonstrated with rawlinseed oil, using an initial amount of 0.02% and an oil addition during cultivation. Nilered staining showed the accumulation of fatty acids inside cells grown in the presenceof oil. In conclusion, each tested vegetable oil was used as carbon and energysource by A. melanogenum. The results indicated that stand linseed oil provides lesscarbon and energy than olive and raw linseed oil. This research is a fundamental stepin unraveling the effects of vegetable oils on biofinish formation.