The agrifood sector is crucial for achieving global food security and environmental sustainability. In the Netherlands, innovations in food technology and adjacent areas are achieved in attractive projects at Universities of Applied Sciences (UASs) in close interaction with government, industry, other knowledge institutes and society. By providing students central positions in innovative joint efforts that answer to the demands of small and medium sized enterprises, the curricula stay up to date and appealing. Examples of such efforts are the Food Innovation Academy (FIA), the World Horti Centre (WHC) and the Food Innovation Community Amsterdam (FICA). Interdisciplinary projects in these settings help to encourage students to choose for a future in agrifood. Exposure is key to reach the target groups. For that reason, several paths on the roadmap of the human capital agenda have to be taken. We developed inspiring learning materials that appeal to students and teachers in secondary schools. A “Genomics Cookbook” to introduce biological knowledge behind nutrigenomics and a velcro-model called “DNAbAND” to explain principles behind the Polymerase Chain Reaction for food safety applications, are examples. These are ways to increase influx into green colleges and universities, and thereby efflux to the agrifood sector.
Het doel van deze studie was het testen van een dertigtal familieleden op Charcot-Marie-Tooth type 1A met behulp van een real time kwantitatieve polymerase kettingreactie. Duplicatie van het gen werd bij 50 % van de familieleden gevonden, overeenkomend met Mendeliaanse overerving.
To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture times for detection of Staphylococci directly from blood culture material. A real-time PCR duplex assay was used to compare 6 different DNA isolation protocols on two different blood culture systems. Negative blood culture material was spiked with MRSA. Bacterial DNA was isolated with: automated extractor EasyMAG (3 protocols), automated extractor MagNA Pure LC (LC Microbiology Kit MGrade), a manual kit MolYsis Plus, and a combination between MolYsis Plus and the EasyMAG. The most optimal isolation method was used to evaluate reduced bacterial culture times. Bacterial DNA isolation with the MolYsis Plus kit in combination with the specific B protocol on the EasyMAG resulted in the most sensitive detection of S.aureus, with a detection limit of 10 CFU/ml, in Bact/ALERT material, whereas using BACTEC resulted in a detection limit of 100 CFU/ml. An initial S.aureus load of 1 CFU/ml blood can be detected after 5 hours of culture in Bact/ALERT3D by combining the sensitive isolation method and the tuf LightCycler assay.
