Waarom gaan mensen naar festivals? Hoe beleven ze een festival? Waarom komen ze wel of niet terug? Hoe kunnen festivalorganisatoren de motivatie en beleving van bezoekers effectief beïnvloeden? Wat betekenen sociale media voor de festivalbeleving? Antwoorden op deze vragen helpen festivalorganisatoren een uniek festival aan te bieden en effectiever resultaten te behalen en overtuigender te rapporteren naar subsidieverstrekkers en sponsors. Het Crossmedialab, onderdeel van het Kenniscentrum Communicatie & Journalistiek van de Hogeschool Utrecht, heeft onderzoek uitgevoerd naar festivalbeleving. Dit cahier geeft een overzicht van onder zochte theorieën en bevat een integraal overzicht van factoren die van invloed zijn op de festivalbeleving. Nieuwe inzichten en het uniek ontwikkelde model van festivalbeleving biedt onderzoekers, eventprofessionals en vakdocenten kansen voor verder onderzoek en praktische toepassing.
Psoriasis (Pso) is a chronic inflammatory skin disease, and up to 30% of Pso patients develop psoriatic arthritis (PsA), which can lead to irreversible joint damage. Early detection of PsA in Pso patients is crucial for timely treatment but difficult for dermatologists to implement. We, therefore, aimed to find disease-specific immune profiles, discriminating Pso from PsA patients, possibly facilitating the correct identification of Pso patients in need of referral to a rheumatology clinic. The phenotypes of peripheral blood immune cells of consecutive Pso and PsA patients were analyzed, and disease-specific immune profiles were identified via a machine learning approach. This approach resulted in a random forest classification model capable of distinguishing PsA from Pso (mean AUC = 0.95). Key PsA-classifying cell subsets selected included increased proportions ofdifferentiated CD4+CD196+CD183-CD194+ and CD4+CD196-CD183-CD194+ T-cells and reduced proportions of CD196+ and CD197+ monocytes, memory CD4+ and CD8+ T-cell subsets and CD4+ regulatory T-cells. Within PsA, joint scores showed an association with memory CD8+CD45RACD197- effector T-cells and CD197+ monocytes. To conclude, through the integration of in-depth flow cytometry and machine learning, we identified an immune cell profile discriminating PsA from Pso. This immune profile may aid in timely diagnosing PsA in Pso.
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Dark homogenous fungal-based layers called biofinishes and vegetable oils are keyingredients of an innovative wood protecting system. The aim of this study was todetermine which of the vegetable oils that have been used to generate biofinishes onwood will provide carbon and energy for the biofinish-inhabiting fungus Aureobasidiummelanogenum, and to determine the effect of the oil type and the amount of oil on thecell yield. Aureobasidium melanogenum was cultivated in shake flasks with differenttypes and amounts of carbon-based nutrients. Oil-related total cell and colony-formingunit growth were demonstrated in suspensions with initially 1% raw linseed,stand linseed, and olive oil. Oil-related cell growth was also demonstrated with rawlinseed oil, using an initial amount of 0.02% and an oil addition during cultivation. Nilered staining showed the accumulation of fatty acids inside cells grown in the presenceof oil. In conclusion, each tested vegetable oil was used as carbon and energysource by A. melanogenum. The results indicated that stand linseed oil provides lesscarbon and energy than olive and raw linseed oil. This research is a fundamental stepin unraveling the effects of vegetable oils on biofinish formation.
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Implanting biocompatible materials is nothing new, 3D printing of cells and extracellular matrix is well underway so growing replacement tissues in a lab is within reach. However, certain obstacles remain: How to culture functional tissues with robust and reproducible 3D architecture? Application of support structures can aid, but what if such scaffolds obstruct functionality of the graft while having limited chance of being degraded within the recipient’s body? Bioplastics are polymers of natural origin that can be degraded enzymatically. We want to use bioplastics for production of 3D printed mesh scaffolds that support cell adhesion, proliferation, differentiation, and maturation (Fig. 1). These scaffolds are designed to be temporal and sacrificial: enzymes will be used to remove the scaffold in a tissue friendly manner prior to implantation allowing tailor made, functional and ideally ‘self-only’ grafts.
